WebCrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping. Nature Methods. PMID 28650476 DOI: 10.1038/Nmeth.4343 : 0.483: 2024: Trigg S, Garza R, MacWilliams A, Nery JR, Bartlett A, Castanon R, Goubil A, Feeney J, O'Malley R, Huang SC, Zhang Z, Galli M, Ecker JR. CrY2H-seq interactome screening Protocol Exchange. WebNov 20, 2024 · A very recent study developed a CrY2H system for all-by-all high-throughput PPI screening. Instead of fusing barcodes for bait and prey genes, this system could directly link the interacting bait and prey pairs after screening ( 11 ). All of the PCR products of fused bait and prey genes were then randomly sheared and subjected to sequencing.
CrY2H-seq: a massively multiplexed assay for deep-coverage …
WebHere we describe a massively-multiplexed yeast two-hybrid method, CrY2H-seq, for deep coverage interactome mapping. This protocol accompanies Trigg et al (Nature Methods … WebCrY2H-seq screens each testing 3.6 million interaction combinations, and reporting a deep coverage network of 8,577 interactions among 1,453 transcription factors, we … board meeting quorum
Development and application of a recombination-based library …
WebJun 26, 2024 · More information: Shelly A Trigg et al, CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping, Nature Methods (2024). DOI: 10.1038/nmeth.4343 Journal information: Nature ... WebJun 26, 2024 · CrY2H-seq, a Cre recombinase reporter-mediated yeast two-hybrid method coupled with next-generation sequencing, enables ultra-high-throughput screening of … Metrics - CrY2H-seq: a massively multiplexed assay for deep-coverage … Supplementary Figure 11 Comparing CrY2H-seq to The Array-Based Ht-Y2h … Supplementary Figure 4 Determining Optimal Sequencing Coverage and … Supplementary Figure 6 Homodimers Do Not Yield Ad-Db PCR Products From … Supplementary Figure 2 Comparing Diploid Strains Y8930/Y8800 and … Supplementary Figure 1 Pilot CrY2H-seq Experiments to Confirm Strain and … Supplementary Figure 14 CrY2H-seq Shows Reduced Screening Costs and … Supplementary Figure 13 Intra- and Interfamily Interactions in AtTFIN-1 - … Supplementary Figure 12 AtTFIN-1 is Enriched for Biologically Relevant … Supplementary Figure 9 Determining wNAPPA Z-Score Threshold - CrY2H … WebApr 24, 2011 · We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a … cliff notes for pride and prejudice