Webted onto HybondTM-N+ membrane (Amersham Phar-macia Biotech) by capillary transfer. The DNA was cross-linked to the membrane by UV irradiation. South-ern hybridization was performed according to “The DIG System User’s Guide for Filter Hybridization” (Boehringer Mannhein). DIG-labeled tctrx gene was used as probe for hybridization assay. Web15 mei 2006 · Genomic DNA (2 mg) from wild type and SHG transgenic plants were digested with EcoRIM, separated by electrophoresis in a 1% agarose green, and transferred at Hybond-N+ film (Amersham Pharmacia). A radiolabeled probe (0.7-kb) specific for the GFP sequence was used in hybridization ( Illustrate 1A ) by a accident priming method …
On the occurrence of thioredoxin in Trypanosoma cruzi
Web9 sep. 2014 · Small and total RNAs were extracted using Trizol as described previously 6 from frozen mycelia in liquid nitrogen. 25-35 μg of sRNAs were separated by … WebHybond N+ is a positively charged nylon membrane capable of high sensitivity in DNA and RNA blotting. Positively charged nylon membrane with a binding capacity for nucleic … book on paradise fire
Nucleic acid labelling detection and membranes
WebHybond-N+ filters (Amersham) (10). Hybridization. (i) Oligonucleotide probes. Oligonucleotide 1818 was labeled with 32P at its 5' end with phage T4 polynucleotide … Web9 mei 2012 · The invention discloses a method for preparing a hybond-N plus surface cross-linking chitosan modified membrane material, which belongs to the technical field … WebAmersham RPN303B Hybond-N+ Nylon Membrane 30cm x 3m Palight ProjectPVC 159837 Palight .236 Red ST-50 24X48 1 1/16 Width 7/8 Bore Aluminum Double Wide 1-5/8 OD Climax Metal D2C-087-A Two-Piece Clamping Collar book on paul newman