Pbr9_mcherry_cre
http://www.zfish.cn/Article/1399.html Splet29. nov. 2010 · The previous limitations of the existing Cre driver lines Tg(hsp70l:Cre) and Tg(hsp70l:EGFP-Cre) should now be resolved with our new Tg(hsp70l:mCherry-T2A-Cre ER T2) alleles. We demonstrate that in the absence of TAM, no non-conditional recombination can be observed at permissive temperatures, a prerequisite for controlled experiments.
Pbr9_mcherry_cre
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Splet什么是CreERT2 ？. 将人雌激素受体（estrogen receptor，简称ER）的配体结合区（LBD）与Cre 重组酶相融合，形成定位于胞浆中的融合蛋白（Cre-ER）。. 只有在雌激素诱导后，融 … http://aammt.tmmu.edu.cn/Upload/rhtml/201502106.htm
Splet03. jun. 2016 · WGA–Cre induced transsynaptic labeling in the cHC was evaluated using a double fluorescent staining for YFP and mCherry (Fig. 3 b, d). In rAAV2/7 WGA–Cre treated animals, YFP + /mCherry − cells were observed in the dentate gyrus and throughout the whole cHC, with a median of 2545 (range 652–4318) cells. Splet12. dec. 1996 · The Cre recombinase of bacteriophage P1 catalyses site-specific recombination between lox-recombination target sites both in prokaryotic and eukaryotic …
SpletmCherry Antibody (M11217) in ICC/IF. Immunofluorescent analysis of mCherry Tag was performed using 70% confluent mCherry-H3 transfected HEK-293 cells. The cells were … SpletWe further generated transgenic zebrafish that express ubiquitous 4-hydroxytamoxifen-controlled Cre recombinase activity from a ubi:cre(ERt2) transgene, as well as ubi:loxP-EGFP-loxP-mCherry (ubi:Switch) transgenics and show their use as a constitutive fluorescent lineage tracing reagent. The ubi promoter and the transgenic lines presented …
Splet15. avg. 1997 · The Cre DNA recombinase of bacteriophage P1 has become a useful tool for precise genomic manipulation in embryonic stem (ES) cells that have been gene modified by homologous recombination. We have re-engineered the cre gene to allow ready identification of living Cre+cells by constructing a function …
SpletCodon-optimized PETase gene was substituted for the mCherry gene of pBR9_mCherry_Cre (resulting in pBR9_PETase_Cre), which is a high-strength expression vector for C. … scuf instinct firmware updateSplet029664 B6N(129S4)- Mafb tm1.1(cre)Kmm /J MafB-mCherry-Cre knock-in mice express a FLAG-tagged-mCherry fluorescent protein and a Cre Recombinase sequence under the … pdf cut off when printingSplethSyn 启动子，也称为 hSyn1 启动子，从人突触素蛋白 1(Synapsin1) 的启动子区克隆得到，长度为 0.5kb 左右，神经元特异表达。. WGA-Cre ， WGA （ Wheat Germ Agglutinin … pdf cut page in halfSplet19. okt. 2024 · To corroborate whether activating the population of MPL SST neurons induces self-grooming, we injected AAV-DIO-ChR2-mCherry (or AAV-DIO-mCherry as the … pdf cubepdf utilitySpletpCMV-Cre-mCherry是碧云天自行研发的含有CMV启动子的用于在哺乳动物细胞中表达带有mCherry (DsRed的突变体，一种非常明亮的红色荧光蛋白)标签的Cre Recombinase (Cre重 … pdf curso vaughanSpletMCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. pdf cube 無料SpletPBARGPE1-mCherry is a filamentous fungus - Escherichia coli shuttle expression vector, which can use gpdA promoter to activate the expression of foreign gene, and can be … scuf infinity xbox one controller