Pcold 1
SpletpCold™ Vector Set: 1 Set: USD $1241.00: Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series … SpletHeating services. Cooling solutions. Cooling products. 24-hour services. Call us for a free estimate.
Pcold 1
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SpletpCold I Vector for cold shock-induced expression in E. coli of a protein with a translation enhancing element followed by a 6xHis tag and a Factor Xa site. Sequence Author: … SpletpCOLADuet-1 Sequence and Map Bacterial vector with a ColA origin for the co-expression of two genes. Bacterial vector with a ColA origin for the co-expression of two genes. …
Splet13. jun. 2014 · aturalSciences)文章编号：1674—2974(2011)lI-0066—04(湖南大学生物学院，湖南：在拟南芥中，Rad23因通过参与泛素／26S白酶体途径调节植物生长发pCold．Rad23融合蛋白重组表达载体，将该载体转化到大肠杆菌BL21中诱导表达目的蛋白．SDS丙烯酰胺凝胶电泳检测结果表明，分子质量为94ku左右的融合重组蛋白在 ... Splet01. sep. 2024 · The pCold-SUMOa plasmid was finally constructed by the (Seamless Cloning Technology) SCT ( Fig. 1 B). pCold-SUMOa contains the same multi cloning sites with pCold TF with the exception of the EcoR I and Pst I, while the SUMO tag contains the latter two restriction sites.
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SpletpCold-R 20-mer / 50% GC 1 binding site. 336 .. 355 = 20 annealed bases T m = 56°C: lacI 3114 .. 4196 = 1083 bp 360 amino acids = 38.6 kDa. Product: lac repressor. The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). ...
http://www.honorgene.com/product_list/carrier_library/122952.html order of embers reputationhttp://bbs.eeworld.com.cn/thread-1239926-1-1.html order of elution gas chromatographySpletOur pCold TF DNA vector is a fusion cold shock expression vector that expresses trigger factor (TF) chaperone as a soluble fusion tag. Trigger factor is a prokaryotic ribosome-associated chaperone protein of 48 kDa which facilitates co-translational folding of nascent polypeptides, reducing protein misfolding and production of insoluble protein. how to transfer photos from samsung a32 to pcSpletCall Procold refrigeration and air conditioning today to experience our expert knowledge and unbeatable service. John 0432 169 420. [email protected]. how to transfer photos from phone to desktopSplet1 : Negative control 2 : T7 Vector 3 : pCold Vector The target protein (kDa) 97.4 66.2 45 31 21.5 14.4 Figure 3. Expression of thermophile gene B was compared with total protein and soluble protein using pCold and T7 Vector followed by CBB staining. T : total protein S : soluble protein Expression level and solubility were improved. T S T S T7 ... how to transfer photos from pixel 3a to pcSpletOur pCold TF DNA vector is a fusion cold shock expression vector that expresses trigger factor (TF) chaperone as a soluble fusion tag. Trigger factor is a prokaryotic ribosome … order of ellery adams booksSplet13. jun. 2004 · Figure 1: Structures of pCold vectors I, II, III and IV. pCold series were derived from backbone plasmid pUC118. M13 IG is the intergenic region of M13 bacteriophage. how to transfer photos from redmi to laptop